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Article Dans Une Revue Chemical Communications Année : 2015

A doubly responsive probe for the detection of Cys4-tagged proteins

Gaëlle Milanole
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Eric Doris
Nathalie Arhel
Thierry Brotin
Jean-Pierre Dutasta

Résumé

Recombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys–Cys–X–X–Cys–Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized 129Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the 129Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association.

Domaines

Chimie Matériaux
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Dates et versions

hal-01187782 , version 1 (17-11-2015)

Identifiants

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Naoko Kotera, Emmanuelle Dubost, Gaëlle Milanole, Eric Doris, Edmond Gravel, et al.. A doubly responsive probe for the detection of Cys4-tagged proteins. Chemical Communications, 2015, 51, pp.11482-11484. ⟨10.1039/C5CC04721H⟩. ⟨hal-01187782⟩
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