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Article Dans Une Revue Pharmaceutical biology Année : 2014

Cytotoxic effects of four Caryophyllaceae species extracts on macrophage cell lines

Résumé

Context: Saponins have been reported to possess antitumor properties, to inhibit angiogenesis and to induce tumor apoptosis. Objective: To test the possible cytotoxic effect of crude extracts from four Caryophyllaceae species including Gypsophila paniculata L., Gypsophila trichotoma Wend., Saponaria officinalis L., and Dianthus sylvestris Wulffen on cultured monocyte/macrophage cell lines. Materials and methods: After acid hydrolysis of the methanol-aqueous extracts, two representative prosaponins of the Caryophyllaceae, gypsogenin 3-O-glucuronide and quillaic acid 3-O-glucuronide were purified using solid-phase extraction (SPE), then identified by ultra-performance liquid chromatography–electrospray/mass spectrometry (UPLC-ESI/MS). Cytotoxic activity of the crude extracts at concentrations ranging from 0.1 to 200 µg/ml was evaluated on rat alveolar macrophage NR8383 and human monocytic THP-1 cell lines. Apoptosis was determined by measuring caspase-3 activity. Results: Quantitative analysis by reversed-phase high-performance liquid chromatography (RP-HPLC) revealed a high content of gypsogenin 3-O-glucuronide in Gypsophila species roots (0.52–1.13% dry weight). At a concentration ≥10 µg/ml of crude extracts, a significant reduction of NR8383 and THP-1 cell lines viability was evidenced using the Trypan blue exclusion test. D. sylvestris extract exhibited the highest toxicity against THP-1 cells. Caspase-3 activation was evidenced after 4 and 24 h incubation of macrophages with 100 µg/ml of S. officinalis and G. trichotoma extracts, indicating apoptosis induction. Discussion and conclusion: Crude extracts from the assayed species revealed cytotoxic effects toward macrophage cell lines. In Gypsophila species, gypsogenin 3-O-glucuronide derivatives could be responsible for the observed cytotoxicity. Therefore, crude extract of Caryophyllaceae is worth investigating for the potential development of agents against cancer cells.
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Dates et versions

hal-01099728 , version 1 (05-01-2015)

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Reneta Gevrenova, Olivier Joubert, Tsvetelina Mandova, Mohamed Zaiou, Yves Chapleur, et al.. Cytotoxic effects of four Caryophyllaceae species extracts on macrophage cell lines. Pharmaceutical biology, 2014, 52 (7), pp.919-925. ⟨10.3109/13880209.2013.868492⟩. ⟨hal-01099728⟩
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