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Communication Dans Un Congrès Année : 2013

Evaluation of the metabolic efficiency of the culture media during fertilization: impact on the development outcomes

Résumé

Introduction: Fertilization processes in mammals are dynamic in nature and highly sensitive to metabolic perturbations. Evidence indicates that embryo culture may adversely affect the developmental potential but it is still difficult to correlate modification of egg functioning according to formula of the culture media. Therefore, in order to measure the functional impact of the culture media, we use the profile of Ca2+ signal dynamics triggered by the sperm as an integrated parameter of egg's activity. Here, the objective is to establish a functionallinkage between the regime of Ca2+ oscillations at fertilization, the composition of the culture media and the postnatal outcomes in the mouse species. Innovation: The innovative aspect of the project lies in the fact that we are using for the first time the self sustained processes of Ca2+ oscillations during fertilization as both a sensor of metabolic activity and as an intracellular signal determining the developmental outcomes. We developed software for analyzing the Ca2+ regime at fertilization following ICSI. We are in position to breakdown every single Ca2+ signal in four elementary phases during the time course of pronuclear formation in orders to detect minute change in the dynamics of Ca2+ release and Ca2+ clearance according to smali changes in the composition of the culture media. Results: The analysis of variance of the number, duration, amplitude and frequency of Ca2+ signais reveals that change in the lactate:pyruvate ratio or exogenous pH profoundly affect the total Ca2+ signal experienced by the egg during the course of PN formation. Hence the total Ca2+ experienced by the egg during fertilization provides a quantitative linkage between the composition of the culture media and the long term impact. lt will facilitate the identification of the molecular actors that influence as earlier as fertilization the animal phenotype.
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Dates et versions

hal-01019154 , version 1 (07-07-2014)

Identifiants

  • HAL Id : hal-01019154 , version 1
  • PRODINRA : 226485

Citer

Jean-Pierre Ozil, Bernadette B. Banrezes, Lionel Berteaux, Thierry Sainte-Beuve, J.M. Cancéla. Evaluation of the metabolic efficiency of the culture media during fertilization: impact on the development outcomes. 1. General Meeting of Epiconcept, Epigenetics and Periconception Environmenment, European Cooperation in Science and Technology (COST). BEL., Apr 2013, Antalya, Turkey. pp.93. ⟨hal-01019154⟩
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