Multi-scale engineering for neuronal cell growth and differentiation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Microelectronic Engineering Année : 2011

Multi-scale engineering for neuronal cell growth and differentiation

Résumé

In this paper we investigate the role of micropatterning and molecular coating for cell culture and differentiation of neuronal cells (Neuro2a cell line) on a polydimethylsiloxane substrate. We investigate arrays of micrometric grooves (line and space) capable to guide neurite along their axis. We demonstrate that pattern dimensions play a major role due to the deformation of the cell occasioned by grooves narrower than typical cell dimension. A technological compromise for optimizing cell density, differentiation rate and neurite alignment has been obtained for 20 lm wide grooves which is a dimension comparable with the average cell dimension. This topographical engineered pattern combined with double-wall carbon nanotubes coating enabled us to obtain adherent cell densities in the order of 104 cells/cm2 and a differentiation rate close to 100%.
Fichier principal
Vignette du fichier
Beduer_8691.pdf (312.01 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-00871244 , version 1 (09-10-2013)

Identifiants

Citer

Amélie Béduer, Laurence Vaysse, Emmanuel Flahaut, Florent Seichepine, Isabelle Loubinoux, et al.. Multi-scale engineering for neuronal cell growth and differentiation. Microelectronic Engineering, 2011, vol. 88 (8), pp. 1668-1671. ⟨10.1016/j.mee.2010.12.049⟩. ⟨hal-00871244⟩
178 Consultations
314 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More