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Article Dans Une Revue Biomedical Microdevices Année : 2012

Monitoring the endocytosis of magnetic nanoparticles by cells using permanent micro-flux sources

Osman Osman
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Marie Frénéa-Robin
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Nora Dempsey
François Buret
Naoufel Haddour

Résumé

Trapping of cells is essential to perform basic handling operations in cell-based microsystems, such as media exchange, concentration, cell isolation and cell sorting. Cell trapping by magnetophoresis typically requires cell labeling with magnetic nanoparticles. Here we report on endocytotic uptake of 100 nm magnetic nanoparticles by Human Embryonic Kidney 293 cells. The attraction of labeled cells by micro-magnet arrays characterised by very high magnetic field gradients (≤10(6) T/m) was studied as a function of labeling conditions (nanoparticle concentration in the extracellular medium, incubation time). The threshold incubation conditions for effective magnetophoretic trapping were established. This simple technique may be exploited to minimise the quantity of magnetic nanoparticles needed for efficient cell trapping, thus reducing stress or nanoparticle-mediated toxicity. Nanoparticle internalization into cells was confirmed using both confocal and Transmission Electron Microscopy (TEM).

Dates et versions

hal-00734036 , version 1 (20-09-2012)

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Citer

Osman Osman, Luiz-Fernando Zanini, Marie Frénéa-Robin, Frédéric Dumas-Bouchiat, Nora Dempsey, et al.. Monitoring the endocytosis of magnetic nanoparticles by cells using permanent micro-flux sources. Biomedical Microdevices, 2012, 14 (5), pp.947-954. ⟨10.1007/s10544-012-9673-4⟩. ⟨hal-00734036⟩
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