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Communication Dans Un Congrès Année : 2012

A New Microfluidic Device for Electric Lysis and Separation of Cells

Mathieu Brun
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Marie Frénéa-Robin
Naoufel Haddour
Anne-Laure Deman
Rosaria Ferrigno

Résumé

This paper demonstrates the potential use of a new microfluidic device embedding thick electrodes for cell lysis and cell separation applications. The system consists of a microfluidic channel featuring conductive walls made of a polydimethylsiloxane (PDMS) matrix mixed with carbon nanoparticles. Cell lysis was performed electrically by applying square pulses across the channel width, which was monitored by fluorimetry. Lysed and unlysed cells showed different dielectrophoretic behavior under appropriate experimental conditions, which suggests that the developed device is suitable to perform both cell lysis and subsequent sorting of viable and dead cells.
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Dates et versions

hal-00734028 , version 1 (20-09-2012)

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Mathieu Brun, Marie Frénéa-Robin, Jean-François Chateaux, Naoufel Haddour, Anne-Laure Deman, et al.. A New Microfluidic Device for Electric Lysis and Separation of Cells. 34th IEEE EMBC, Aug 2012, San Diego, United States. pp.SaA07.4, ⟨10.1109/EMBC.2012.6347430⟩. ⟨hal-00734028⟩
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