Control of the specific growth rate of Bacillus subtilis for the production of biosurfactant lipopeptides in bioreactors with foam overflow - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Process Biochemistry Année : 2010

Control of the specific growth rate of Bacillus subtilis for the production of biosurfactant lipopeptides in bioreactors with foam overflow

Résumé

This paper formulates a feeding law for a bioprocess dedicated to the production of an antibiotic surfactant using Bacillus subtilis. The specificity of the process relies on the use of the surface active property of the product to extract it by foaming. The control law is designed to maintain a constant specific biomass growth rate while taking into account the particularity of the process. This law can be regarded as a generalization of the conventional exponential feeding strategy and is generic enough to encompass the case of continuous processes with partial recycling. Conventional exponential feeding strategies indeed fail to account for the loss of biomass induced by the foaming. Previous experiments have provided a model of the process and values for its parameters. From this information, a feeding rate law was computed using the feeding strategy proposed in this paper and applied to an experimental culture. This experiment allows discussion of the modeling of the biomass extraction method used in this study. The results on the estimated specific growth rate highlight the complete agreement between the expected and experimental features. Further process optimization studies can now be performed on the basis of the constant specific biomass growth rate
Fichier principal
Vignette du fichier
PRBI-D-09-00701R1_Hal.pdf (833.01 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-00711837 , version 1 (01-11-2012)

Identifiants

Citer

Salah Chenikher, Jean Sébastien Guez, François Coutte, Komi Midzodzi Pekpe, Philippe Jacques, et al.. Control of the specific growth rate of Bacillus subtilis for the production of biosurfactant lipopeptides in bioreactors with foam overflow. Process Biochemistry, 2010, 45 (11), pp.1800-1807. ⟨10.1016/j.procbio.2010.06.001⟩. ⟨hal-00711837⟩
279 Consultations
1595 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More