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Communication Dans Un Congrès Année : 2012

Blind deconvolution of 3D data in wide field fluorescence microscopy

Résumé

In this paper we propose a blind deconvolution algorithm for wide field fluorescence microscopy. The 3D PSF is modeled after a parametrized pupil function. The PSF parameters are estimated jointly with the object in a maximum a posteriori framework. We illustrate the performances of our algorithm on experimental data and show significant resolution improvement notably along the depth. Quantitative measurements on images of calibration beads demonstrate the benefits of blind deconvolution both in terms of contrast and resolution compared to non-blind deconvolution using a theoretical PSF.
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Dates et versions

hal-00691249 , version 1 (25-04-2012)

Identifiants

  • HAL Id : hal-00691249 , version 1

Citer

Ferréol Soulez, Loïc Denis, Yves Tourneur, Éric Thiébaut. Blind deconvolution of 3D data in wide field fluorescence microscopy. International Symposium on Biomedical Imaging, May 2012, Barcelone, Spain. pp.CDROM. ⟨hal-00691249⟩
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