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Article Dans Une Revue Nature Immunology Année : 2011

IFIT1 is an antiviral protein that recognises 5'-triphosphate RNA

Résumé

Antiviral innate immunity relies on recognition of microbial structures. One such structure is viral RNA that carries a triphosphate group on its 5'terminus (PPP-RNA). In an affinity proteomics approach with PPP-RNA as bait we identified interferon induced protein with tetratricopeptide repeats 1 (IFIT1) to mediate binding of a larger protein complex containing other IFIT proteins. IFIT1 bound PPP-RNA with nanomolar affinity and required R187 in a highly charged groove. In the absence of IFIT1 growth and pathogenicity of PPP-RNA viruses were severely increased. In contrast, IFITs were dispensable for clearance of pathogens not generating PPP-RNA. Based on this specificity and the high abundance of IFITs we propose that the IFIT complex antagonises viruses by sequestering specific viral nucleic acids.
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Dates et versions

hal-00648046 , version 1 (05-12-2011)

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Giulio Superti-Furga, Andreas Pichlmair, Caroline Lassnig, Christoph L Baumann, Tilmann Bürckstümmer, et al.. IFIT1 is an antiviral protein that recognises 5'-triphosphate RNA. Nature Immunology, 2011, ⟨10.1038/ni.2048⟩. ⟨hal-00648046⟩

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