IFIT1 is an antiviral protein that recognises 5'-triphosphate RNA
Résumé
Antiviral innate immunity relies on recognition of microbial structures. One such structure is viral RNA that carries a triphosphate group on its 5'terminus (PPP-RNA). In an affinity proteomics approach with PPP-RNA as bait we identified interferon induced protein with tetratricopeptide repeats 1 (IFIT1) to mediate binding of a larger protein complex containing other IFIT proteins. IFIT1 bound PPP-RNA with nanomolar affinity and required R187 in a highly charged groove. In the absence of IFIT1 growth and pathogenicity of PPP-RNA viruses were severely increased. In contrast, IFITs were dispensable for clearance of pathogens not generating PPP-RNA. Based on this specificity and the high abundance of IFITs we propose that the IFIT complex antagonises viruses by sequestering specific viral nucleic acids.
Origine | Fichiers produits par l'(les) auteur(s) |
---|
Loading...