Polyelectrolyte microcapsules with entrapped multicellular tumor spheroids as a novel tool to study the effects of photodynamic therapy - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biomedical Materials Research Part A Année : 2011

Polyelectrolyte microcapsules with entrapped multicellular tumor spheroids as a novel tool to study the effects of photodynamic therapy

Résumé

In the current study, semi-permeable alginate-oligochitosan microcapsules for multicellular tumor spheroids (MTS) generation were elaborated and tested, to estimate a response of the microencapsulated MTS (MMTS) to photodynamic therapy (PDT). The microcapsules (mean diameter 600 μm) with entrapped human breast adenocarcinoma MCF-7 cells were obtained using an electrostatic bead generator, and MMTS were generated by in vitro long-term cell cultivation. The formed MMTS were incubated in Chlorin e6 photosensitizer solution and then irradiated using 650-nm laser light. The cell viability was measured by MTT-assay in 24 h after irradiation, and histological analysis was performed. The proposed MTS-based model was found to be more resistant to the PDT than the two-dimensional monolayer cell culture model. Thus, MMTS could be considered as a promising three-dimesional in vitro model to estimate the doses of drugs or parameters for PDT in vitro before carrying out preclinical tests.

Dates et versions

hal-00548926 , version 1 (20-12-2010)

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Daria Zaytseva-Zotova, Olga Udartseva, Elena Andreeva, Artur Bartkowiak, Lina Bezdetnaya, et al.. Polyelectrolyte microcapsules with entrapped multicellular tumor spheroids as a novel tool to study the effects of photodynamic therapy. Journal of Biomedical Materials Research Part A, 2011, 97 (2), pp.255-262. ⟨10.1002/jbm.b.31808⟩. ⟨hal-00548926⟩
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