After the successful fourth edition in 2009, the fifth edition of the Enterprise Integration, Interoperability and Networking workshop (EI2N'2010) has been organised as part of the OTM'2010 Federated Conferences and is supported by the IFAC Technical Committee 5.3 "Enterprise Integration and Networking", the IFIP TC 8 WG 8.1 "Design and Evaluation of Information Systems", the SIG INTEROP Grande-Région on "Enterprise Systems Interoperability" and the French CNRS National Research Group GDR MACS. Collaboration is necessary for enterprises to prosper in the current extreme dynamic and heterogeneous business environment. Enterprise integration, interoperability and networking are the major disciplines that have studied how to do companies to collaborate and communicate in the most effective way. These disciplines are well-established and are supported by international conferences, initiatives, groups, task forces and governmental projects all over the world where different domains of knowledge have been considered from different points of views and a variety of objectives (e.g., technological or managerial). Enterprise Integration involves breaking down organizational barriers to improve synergy within the enterprise so that business goals are achieved in a more productive and efficient way. The past decade of enterprise integration research and industrial implementation has seen the emergence of important new areas, such as research into interoperability and networking, which involve breaking down organizational barriers to improve synergy within the enterprise and among enterprises. The ambition to achieve dynamic, efficient and effective cooperation of enterprises within networks of companies, or in an entire industry sector, requires the improvement of existing, or the development of new, theories and technologies. Enterprise Modelling, Architecture, and semantic techniques are the pillars supporting the achievement of Enterprise Integration and Interoperability. Internet of Things and Cloud Computing now present new opportunities to realize inter enterprise and intra enterprise integration. For these reasons, the workshop's objective is to foster discussions among representatives of these neighbouring disciplines and to discover new research paths within the enterprise integration community. After peer reviews, 6 papers have been accepted out of 12 submissions to this workshop. Prof. Michael Sobolewski (Polish-Japanese Institute of IT, Poland) has been invited as EI2N plenary keynote on "Exerted Enterprise Computing: from Protocol-oriented Networking to Exertion-oriented Networking". In addition to the presentations of the accepted papers, groups have been organised into what E2IN traditionally calls "workshop cafés", to discuss and debate the presented topics. This year discussion enabled putting forward new research related to "interoperability issues in collaborative information systems". These groups reported the results of the respective discussions during a plenary session that was jointly organised with the CoopIS'2010 conference, in order to share the vision for future research on this top domain. The papers published in this volume of proceedings present samples of current research in the enterprise modelling, systems interoperability, services management, cloud integration and, more globally, systems engineering and enterprise architecture domains. Some new architecting principles that has gained currency in the recent past is semantic technique, service oriented architecture and cloud computing with their principles, reference models and technology, and if applied correctly can be an important contributor to the future of interoperable, networked and collaborative enterprises. The success of this complex field also depends on the maturity and coherency of the management of the involved enterprises, a topic covered by the second workshop café. As a special track of EI2N'2010, SeDeS'2010 is the first international workshop on Semantics & Decision Support. The call for papers saw 12 submissions, among which the Programme Committee has selected 4 papers to be presented at EI2N'2010. The selected papers cover the topics of ontology-based decision making applications in the fields of eGovernment, eLearning, business rule management and Human Resource Management.