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Article Dans Une Revue Journal of Mass Spectrometry Année : 2009

Comparison of LID versus CID activation modes in tandem mass spectrometry of peptides

Résumé

We report our contribution to the systematic investigation of peptide fragmentations performed on high-performance Tof equipement operating in MS and MS/MS modes, such as ESI-QqTof and MALDI-Tof/Tof intruments that are commonly available today in proteomic laboratories. Whereas the former analyzer's configuration provides low-energy collision-induced dissociations (CID), the latter allows tunable activation methods of the selected parent ion to induce either metastable laser-induced dissociations (LID) or high-energy CID ('gas on spectra LID'). Fragmentation of the monoprotonated ion of 53 peptides (FW 807-2853 g/mol) was undertaken upon low-energy CID on an ESI-QT of mass spectrometer (Waters) as well as high-energy CID and LID conditions on a MALDI Ultraflex mass spectrometer (Bruker). Systematic comparison of MS/MS spectra provided useful information on the performance of each piece of equipment for efficient peptide sequencing and also insights into the observed fragmentation behaviors.

Domaines

Chimie organique

Dates et versions

hal-00391087 , version 1 (03-06-2009)

Identifiants

Citer

Nawar Shenar, Nicolas Sommerer, Jean Martinez, Christine Enjalbal-Goubet. Comparison of LID versus CID activation modes in tandem mass spectrometry of peptides. Journal of Mass Spectrometry, 2009, 44 (5), pp.621-632. ⟨10.1002/jms.1535⟩. ⟨hal-00391087⟩
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