The Generalized Traveling Salesman Problem (GTSP) is a generalization of the well-known Traveling Salesman Problem (TSP), in which the set of nodes is divided into mutually exclusive clusters. The objective of the GTSP consists in visiting each cluster exactly once in a tour, while minimizing the sum of the routing costs. This paper addresses the solution of the GTSP using a Memetic Algorithm procedure. The originality of our approach rests on the crossover procedure that uses a large neighborhood search. This algorithm is compared with other algorithms on a set of 41 standard test problems with up to 442 nodes. The obtained results show that our algorithm is efficient in both solution quality and computation time.