In vitro induction of differentiation by retinoic acid in an immortalized olfactory neuronal cell line
Sophie Lakard
(1, 2, 3)
,
Eric Lesniewska
(4, 5)
,
Germaine Michel
(3)
,
Boris Lakard
(1, 2)
,
Nadege Morrand-Villeneuve
(3)
,
Claudine Versaux-Botteri
(3)
1
LCMI -
Laboratoire de Chimie des Matériaux et Interfaces (EA 474)
2 UTINAM - Univers, Théorie, Interfaces, Nanostructures, Atmosphère et environnement, Molécules (UMR 6213)
3 NEURO - Laboratoire de Neurosciences Intégratives et Cliniques - UFC (UR 481)
4 ICB - Laboratoire Interdisciplinaire Carnot de Bourgogne
5 LPUB - Laboratoire de Physique de l'Université de Bourgogne
2 UTINAM - Univers, Théorie, Interfaces, Nanostructures, Atmosphère et environnement, Molécules (UMR 6213)
3 NEURO - Laboratoire de Neurosciences Intégratives et Cliniques - UFC (UR 481)
4 ICB - Laboratoire Interdisciplinaire Carnot de Bourgogne
5 LPUB - Laboratoire de Physique de l'Université de Bourgogne
Résumé
In this study, we used a neuronal cell line generated by transfection of rat olfactory epithelium with immortalizing recombinant oncogene E1A of adenovirus-2. The resulting 13.S.1.24 line of transformed cells expressed an antigenic phenotype of olfactory neuronal progenitors. Time-dependency assessments over 1 week of treatment indicated that apoptosis and differentiation induced by retinoic acid (RA) were concomitant. Indeed, RA altered the cell proliferation rate, but it also stimulated differentiation of surviving 13.S.1.24 cells into bipolar olfactory marker protein-immunoreactive neurons. To characterize the nature of the cells we used immunocytochemistry, optical imaging, scanning electron microscopy and atomic force microscopy.
Domaines
Neurosciences [q-bio.NC]Format du dépôt | Notice |
---|---|
Type de dépôt | Article dans une revue |
Résumé |
en
In this study, we used a neuronal cell line generated by transfection of rat olfactory epithelium with immortalizing recombinant oncogene E1A of adenovirus-2. The resulting 13.S.1.24 line of transformed cells expressed an antigenic phenotype of olfactory neuronal progenitors. Time-dependency assessments over 1 week of treatment indicated that apoptosis and differentiation induced by retinoic acid (RA) were concomitant. Indeed, RA altered the cell proliferation rate, but it also stimulated differentiation of surviving 13.S.1.24 cells into bipolar olfactory marker protein-immunoreactive neurons. To characterize the nature of the cells we used immunocytochemistry, optical imaging, scanning electron microscopy and atomic force microscopy.
|
Titre |
en
In vitro induction of differentiation by retinoic acid in an immortalized olfactory neuronal cell line
|
Auteur(s) |
Sophie Lakard
1, 2, 3
, Eric Lesniewska
4, 5
, Germaine Michel
3
, Boris Lakard
1, 2
, Nadege Morrand-Villeneuve
3
, Claudine Versaux-Botteri
3
1
LCMI -
Laboratoire de Chimie des Matériaux et Interfaces (EA 474)
( 9989 )
- 16 route de Gray 25030 Besançon Cedex
- France
2
UTINAM -
Univers, Théorie, Interfaces, Nanostructures, Atmosphère et environnement, Molécules (UMR 6213)
( 85871 )
- 16, route de Gray 25030 BESANCON CEDEX France
- France
3
NEURO -
Laboratoire de Neurosciences Intégratives et Cliniques - UFC (UR 481)
( 116048 )
- 19 rue Ambroise Paré,
Bâtiment Rabelais,
25030 Besançon Cedex
- France
4
ICB -
Laboratoire Interdisciplinaire Carnot de Bourgogne
( 103192 )
- Université de Bourgogne - 9 avenue Alain Savary - BP 47870 - 21078 Dijon Cedex
- France
5
LPUB -
Laboratoire de Physique de l'Université de Bourgogne
( 553 )
- Université de Bourgogne - 9 Av Alain Savary - BP 47870 21078 DIJON CEDEX
- France
|
Comité de lecture |
Oui
|
Vulgarisation |
Non
|
Langue du document |
Anglais
|
Nom de la revue |
|
Audience |
Non spécifiée
|
Date de publication |
2007
|
Volume |
109
|
Numéro |
2
|
Page/Identifiant |
111-121
|
Domaine(s) |
|
Mots-clés |
en
Olfactory neuronal cells, Cell culture, Immunocytochemistry, Atomic force microscopy, Scanning electron microscopy
|
DOI | 10.1016/j.acthis.2006.10.001 |
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