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Article dans une revue Acta Histochemica Année : 2007

In vitro induction of differentiation by retinoic acid in an immortalized olfactory neuronal cell line

Résumé

In this study, we used a neuronal cell line generated by transfection of rat olfactory epithelium with immortalizing recombinant oncogene E1A of adenovirus-2. The resulting 13.S.1.24 line of transformed cells expressed an antigenic phenotype of olfactory neuronal progenitors. Time-dependency assessments over 1 week of treatment indicated that apoptosis and differentiation induced by retinoic acid (RA) were concomitant. Indeed, RA altered the cell proliferation rate, but it also stimulated differentiation of surviving 13.S.1.24 cells into bipolar olfactory marker protein-immunoreactive neurons. To characterize the nature of the cells we used immunocytochemistry, optical imaging, scanning electron microscopy and atomic force microscopy.
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Dates et versions

hal-00187063, version 1 (13-11-2007)

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Sophie Lakard, Eric Lesniewska, Germaine Michel, Boris Lakard, Nadege Morrand-Villeneuve, et al.. In vitro induction of differentiation by retinoic acid in an immortalized olfactory neuronal cell line. Acta Histochemica, 2007, 109 (2), pp.111-121. ⟨10.1016/j.acthis.2006.10.001⟩. ⟨hal-00187063⟩
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