Protein detection based on microelectrodes with the PPy[3,3-Co(1,2-C2B9H11)](2) solid contact and immobilized proteinases: Preliminary investigations - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Materials Science and Engineering: C Année : 2006

Protein detection based on microelectrodes with the PPy[3,3-Co(1,2-C2B9H11)](2) solid contact and immobilized proteinases: Preliminary investigations

S. V. Verevka
  • Fonction : Auteur
Sergei V. Dzyadevych
  • Fonction : Auteur
N. Zine
  • Fonction : Auteur
J. Bausells
  • Fonction : Auteur
Josep Samitier
  • Fonction : Auteur
  • PersonId : 839307
Abdelhamid Errachid
  • Fonction : Auteur

Résumé

Protein selective potentiometric microelectrodes with the PPy[3,3-Co(1,2-C2B9H11)(2) solid internal contact were fabricated by the immobilization of different proteinases such as trypsin, pronase E and carboxypeptidase B on the surface of platinum microelectrodes modified by a conducting polymer. The performance of these biosensors was investigated by using potentionnetric measurements. The feasibility of this biosensor for protein determination was demonstrated, with a detection limit of about 1-2 mu g/ml for prolamine in buffer solution.
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Dates et versions

hal-00141450 , version 1 (12-04-2007)

Identifiants

  • HAL Id : hal-00141450 , version 1

Citer

O. A. Biloivan, S. V. Verevka, Sergei V. Dzyadevych, Nicole Jaffrezic-Renault, N. Zine, et al.. Protein detection based on microelectrodes with the PPy[3,3-Co(1,2-C2B9H11)](2) solid contact and immobilized proteinases: Preliminary investigations. Materials Science and Engineering: C, 2006, 26 (2-3 Sp. Iss. SI), pp.574-577. ⟨hal-00141450⟩
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