Study of mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile and their application for immunosensor engineering - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biosensors and Bioelectronics Année : 2004

Study of mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile and their application for immunosensor engineering

Yanxia X. Hou
C. Tlili
  • Fonction : Auteur
Aidong D. Zhang
  • Fonction : Auteur
Abdelhamid Errachid
  • Fonction : Auteur
Josep Samitier
  • Fonction : Auteur
  • PersonId : 839307
J. Bausells
  • Fonction : Auteur

Résumé

Langmuir-Blodgett (LB) technique appears to be quite suitable for generating biospecific surfaces and it has potential application for fabricating biosensors. In this work, mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile have been transferred onto hydrophobic silver surface previously modified by I-octadecanethiol (ODT) SAMs. In order to obtain stable LB films, the influences of different parameters - type of amphiphile, surface pressure and pH - on the properties of mixed IgG/amphiphile monolayer. were investigated. Electrochemical properties of the engineered immunosensor have been measured by impedimetric spectroscopy. The immunosensor obtained exhibits a high sensitivity and a good specificity in a linear dynamic range from 200 to 1000 ng ml(-1). (C) 2004 Elsevier B.V. All rights reserved.
Fichier non déposé

Dates et versions

hal-00140629 , version 1 (07-04-2007)

Identifiants

  • HAL Id : hal-00140629 , version 1

Citer

Yanxia X. Hou, C. Tlili, Nicole Jaffrezic-Renault, Aidong D. Zhang, Claude Martelet, et al.. Study of mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile and their application for immunosensor engineering. Biosensors and Bioelectronics, 2004, 20 (6), pp.1126-1133. ⟨hal-00140629⟩
47 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More