An efficient procedure for purification of the obligate intracellular Wolbachia pipientis and representative amplification of its genome by multiple displacement amplification - Archive ouverte HAL Access content directly
Journal Articles Applied and Environmental Microbiology Year : 2005

An efficient procedure for purification of the obligate intracellular Wolbachia pipientis and representative amplification of its genome by multiple displacement amplification

Abstract

procedure to representatively amplify Wolbachia genome by multiple displacement amplification from limited infected host tissue (0.2 g or 2 x 107 cell line). We obtained sufficient amounts (8 to 10 µg) of DNA of suitable quality for genomic studies with which we demonstrated that the amplified DNA contained all of the Wolbachia loci targeted. In addition, our data indicated that the genome of wRi strain, an obligatory endosymbiont of Drosophila simulans, shared similar overall architecture with its relative strain wMel.
No file

Dates and versions

hal-00023397 , version 1 (13-11-2006)

Identifiers

  • HAL Id : hal-00023397 , version 1
  • PRODINRA : 251979

Cite

Patrick Mavingui, Van Tran Van, Estelle Labeyrie, Edwige Rancès, Fabrice Vavre, et al.. An efficient procedure for purification of the obligate intracellular Wolbachia pipientis and representative amplification of its genome by multiple displacement amplification. Applied and Environmental Microbiology, 2005, 71, pp.6910-6917. ⟨hal-00023397⟩
100 View
0 Download

Share

Gmail Facebook X LinkedIn More