A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cell Reports Année : 2018

A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection

Résumé

Heterochromatin plays a central role in the process of immune evasion, pathogenesis, and transmission of the malaria parasite Plasmodium falciparum during blood stage infection. Here, we use ChIP sequencing to demonstrate that sporozoites from mosquito salivary glands expand heterochromatin at subtelomeric regions to silence blood-stage-specific genes. Our data also revealed that heterochromatin enrichment is predictive of the transcription status of clonally variant genes members that mediate cytoadhesion in blood stage parasites. A specific member (here called NF54varsporo) of the var gene family remains euchromatic, and the resultant PfEMP1 (NF54_SpzPfEMP1) is expressed at the sporozoite surface. NF54_SpzPfEMP1-specific antibodies efficiently block hepatocyte infection in a strain-specific manner. Furthermore, human volunteers immunized with infective sporozoites developed antibodies against NF54_SpzPfEMP1. Overall, we show that the epigenetic signature of var genes is reset in mosquito stages. Moreover, the identification of a strain-specific sporozoite PfEMP1 is highly relevant for vaccine design based on sporozoites.
Fichier principal
Vignette du fichier
mmc8.pdf (6.49 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

pasteur-03249869 , version 1 (04-06-2021)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Gigliola Zanghì, Shruthi S. Vembar, Sebastian Baumgarten, Shuai Ding, Julien Guizetti, et al.. A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection. Cell Reports, 2018, 22 (11), pp.2951-2963. ⟨10.1016/j.celrep.2018.02.075⟩. ⟨pasteur-03249869⟩
54 Consultations
55 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More