Increasing genomic information in bivalves through new EST collections in four species: Development of new genetic markers for environmental studies and genome evolution
Arnaud Tanguy
(1)
,
Nicolas Bierne
(2)
,
Carlos Saavedra
(3)
,
Benjamin Pina
(4)
,
Evelyne Bachère
(5)
,
Michael Kube
(6)
,
Eric Bazin
(2)
,
François Bonhomme
(1)
,
Pierre Boudry
(7)
,
Viviane Boulo
(8, 5)
,
Isabelle Boutet
(1)
,
Leonor M. Cancela
(9)
,
Carole Dossat
(10)
,
Pascal Favrel
(11)
,
Arnaud Huvet
(11)
,
Sergio Jarque
(4)
,
Didier Jollivet
(1, 12)
,
Sven Klages
(6)
,
Sylvie Lapegue
(7)
,
Ricardo Leite
(9)
,
Jeanne Moal
(11)
,
Dario Moraga
(13)
,
Richard Reinhardt
(6)
,
Jean-François Samain
(11)
,
Eleftherios Zouros
(14)
,
Adelino Canario
(15)
1
SBR -
Station biologique de Roscoff [Roscoff]
2 UMR ISEM - Institut des Sciences de l'Evolution de Montpellier
3 IATS - Instituto de Acuicultura de Torre la Sal
4 IBMB-CSIC
5 GPIA - Génome, populations, interactions, adaptation
6 MPIMG - Max Planck Institute for Molecular Genetics
7 AGSAE - Unité Amélioration génétique, Santé animale et Environnement
8 LEADNC - Unité Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle-Calédonie
9 CCMAR - Centre of Marine Sciences [Faro]
10 GENOSCOPE - Genoscope - Centre national de séquençage [Evry]
11 PE2M - Physiologie et Ecophysiologie des Mollusques Marins
12 AD2M - Adaptation et diversité en milieu marin
13 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
14 Department of Biology
15 CCMAR - Centro de Ciências do Mar [Faro]
2 UMR ISEM - Institut des Sciences de l'Evolution de Montpellier
3 IATS - Instituto de Acuicultura de Torre la Sal
4 IBMB-CSIC
5 GPIA - Génome, populations, interactions, adaptation
6 MPIMG - Max Planck Institute for Molecular Genetics
7 AGSAE - Unité Amélioration génétique, Santé animale et Environnement
8 LEADNC - Unité Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle-Calédonie
9 CCMAR - Centre of Marine Sciences [Faro]
10 GENOSCOPE - Genoscope - Centre national de séquençage [Evry]
11 PE2M - Physiologie et Ecophysiologie des Mollusques Marins
12 AD2M - Adaptation et diversité en milieu marin
13 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
14 Department of Biology
15 CCMAR - Centro de Ciências do Mar [Faro]
Nicolas Bierne
- Fonction : Auteur
- PersonId : 170491
- IdHAL : nbierne
- ORCID : 0000-0003-1856-3197
- IdRef : 067120458
Pierre Boudry
- Fonction : Auteur
- PersonId : 13840
- IdHAL : pierre-boudry
- ORCID : 0000-0002-5150-2276
- IdRef : 079120741
Viviane Boulo
- Fonction : Auteur
- PersonId : 1327248
- IdHAL : viviane-boulo
- ORCID : 0000-0002-0479-8406
Isabelle Boutet
- Fonction : Auteur
- PersonId : 753183
- IdHAL : isabelle-boutet
- ORCID : 0000-0003-0619-9316
Arnaud Huvet
- Fonction : Auteur
- PersonId : 182303
- IdHAL : arnaud-huvet
- ORCID : 0000-0001-6912-881X
- IdRef : 132891778
Didier Jollivet
- Fonction : Auteur
- PersonId : 908687
- IdHAL : didier-jollivet
- ORCID : 0000-0001-8042-1857
Sylvie Lapegue
- Fonction : Auteur
- PersonId : 866511
Dario Moraga
- Fonction : Auteur
- PersonId : 181933
- IdHAL : dario-moraga
- IdRef : 080806287
Résumé
The generation of EST information is an essential step in the genomic characterisation of species. In the context of the European Network Marine Genomics, a common goal was to significantly increase the amount of ESTs in commercial marine mollusk species and more specifically in the less studied but ecologically and commercially important groups, such as mussel and clam genera. Normalized cDNA libraries were constructed for four different relevant bivalves species (Crassostrea gigas, Mytilus edulis, Ruditapes decussatus and Bathymodiolus azoricus), using numerous tissues and physiological conditions. In this paper, we present the analysis of the 13,013 expressed sequence tags (ESTs) generated. Each EST library was independently assembled and 1300–3000 unique sequences were identified in each species. For the different species, functional categories could be assigned to only about 16 to 27% of ESTs using the GO annotation tool. All sequences have been incorporated into a publicly available database and form the basis for subsequent microarray design, SNP detection and polymorphism analysis, and the placement of novel markers on genetic linkage maps.