The colonization dynamics of the black tru‚e in an arti®cial ®eld were assessed through analyses of microsatellite and RAPD markers. The tru‚e ®eld was composed of three tree species and mycelial inoculum of three di€erent origins, and was monitored for the ®rst three years of tru‚e production. We found very low levels of genetic diversity. Isolation by distance was detected only at the between- tree level. This could be interpreted as local colonization around each tree facilitated by the presence of the tree root system. At the larger spatial scale of the European range, the absence of isolation by distance corroborates the hypothesis of an impact of glaciation on genetic variation, followed by rapid postglaciation demographic expansion. In addition, genetic variation of harvested tru‚es was explained by neither inoculation origin, nor tree species. Our study questions the real impact of man- made inoculation of tree root systems with fungal mycelia.