Article Dans Une Revue Nature Immunology Année : 2025

DC subsets and states unraveled across human juxtatumoral and malignant tissues

Lizhi He
  • Fonction : Auteur
Timothy Wiggins
  • Fonction : Auteur
Jiang He
  • Fonction : Auteur
George Emanuel
  • Fonction : Auteur

Résumé

Dendritic cells (DCs) are professional antigen-presenting cells. While plasmacytoid DCs (pDCs) are poor antigen-presenting cells at steady state, myeloid DCs (mDCs), which include DC1s, DC2s and DC3s, are specialized in T cell priming. To generate unbiased human DC atlases, we integrated DCs from 13 tumor tissues across 40 datasets to create a pDC + mDC-VERSE (DC-VERSE) and an mDC-VERSE single-cell RNA-sequencing compendium. We characterized DC subsets and 'states' across these tissues. Most studied tumors contained CD207+ DCs, a subset of CD1c+ DCs, whose expansion inversely correlated with tumor CD8+ resident memory T cells, T cell clonality and the survival of patients treated with immune checkpoint inhibitors. Similarly to CCR7+ mDCs (a common state of DC1s, DC2s and DC3s), we found that CD207+ DCs were a common state of DC2s and DC3s. Spatially resolved single-cell transcriptomic and immunohistofluorescence analyses of human carcinomas demonstrated that lymphocytes and most DCs were enriched within the tumor stroma, while CD207+ DCs were mostly embedded within tumor nests. These DC-VERSEs provide a robust resource available to the scientific community on DCs in health and pathology.

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hal-05593673 , version 1 (16-04-2026)

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Kevin Mulder, Margaux Gardet, Wan Ting Kong, Amit Ashok Patel, Anne Calvez, et al.. DC subsets and states unraveled across human juxtatumoral and malignant tissues. Nature Immunology, 2025, 27 (1), pp.135-149. ⟨10.1038/s41590-025-02337-x⟩. ⟨hal-05593673⟩
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