Regulation of NUDX1-1a gene, a pivotal player in geraniol production in Roses
Résumé
Rose has been used since antiquity for its ornament and scent qualities. Rose scent originates from a complex mixture of volatile organic compounds (VOCs), geraniol being one if its main constituents. Contrary to other aromatic plants, geraniol in roses is synthetized by a non-canonical pathway involving a different enzyme family, the nudix hydrolases. Geraniol biosynthesis is not only related to key synthases but is also regulated by the activity of transcription factors (TFs). Some TFs have been identified to modulate NUDX1-1a gene, but its positive and tissue specific regulation remains unexplored. In the present study, a petal-specific TF was sought for, performing a comparative transcriptome analysis of different tissues, stages and varieties of roses. The action of the TF candidates on the promoter of the target gene NUDX1-1a was studied using tobacco dual-luciferase assays, yeast one-hybrid (Y1H) analysis and CHIP-qPCR. We determinated that one particular TF was able to transactivate the expression of the reporter gene. This result gives insights related to the transcriptional regulatory framework underlying the control of geraniol production in rose.
| Origine | Fichiers produits par l'(les) auteur(s) |
|---|---|
| Licence |