Article Dans Une Revue Nature Genetics Année : 2025

Uniform dynamics of cohesin-mediated loop extrusion in living human cells

Résumé

Most animal genomes are partitioned into topologically associating domains (TADs), created by cohesin-mediated loop extrusion and defined by convergently oriented CCCTC-binding factor (CTCF) sites. The dynamics of loop extrusion and its regulation remain poorly characterized in vivo. Here we tracked the motion of TAD anchors in living human cells to visualize and quantify cohesin-dependent loop extrusion across multiple endogenous genomic regions. We show that TADs are dynamic structures whose anchors are brought in proximity about once per hour and for 6–19 min (~16% of the time). Moreover, TADs are continuously extruded by multiple cohesin complexes. Remarkably, despite strong differences in Hi-C patterns across chromatin regions, their dynamics is consistent with the same density, residence time and speed of cohesin. Our results suggest that TAD dynamics is primarily governed by the location and affinity of CTCF sites, enabling genome-wide predictive models of cohesin-dependent chromatin interactions.

Fichier principal
Vignette du fichier
Sabate_Lelandais_Uniform_dynamics_of_cohesin-mediated_loop.pdf (25.51 Mo) Télécharger le fichier
Origine Publication financée par une institution
Licence
DOI

Cite 10.5281/zenodo.16949930 Jeu de données Sabaté, T., Lelandais, B., Robert, M.-C., Szalay, M., Tinevez, J.-Y., Bertrand, E., & Zimmer, C. (2025). Uniform dynamics of cohesin-mediated loop extrusion in living human cells [Data set]. Zenodo. https://doi.org/10.5281/ZENODO.16949930

Dates et versions

hal-05527371 , version 1 (25-02-2026)

Licence

Identifiants

Citer

Thomas Sabaté, Benoît Lelandais, Marie-Cécile Robert, Michael Szalay, Jean-Yves Tinevez, et al.. Uniform dynamics of cohesin-mediated loop extrusion in living human cells. Nature Genetics, 2025, 57 (12), pp.3152-3164. ⟨10.1038/s41588-025-02406-9⟩. ⟨hal-05527371⟩
158 Consultations
21 Téléchargements

Altmetric

Partager

  • More