Article Dans Une Revue Science Année : 2026

Recruitment of bifunctional regulator thermospermine to methylated ribosomes directs xylem fate

Raili Ruonala
Eva Hellmann
Huili Liu
Anders Haakonsson
Nuria de Diego
Mariia Shcherbii
  • Fonction : Auteur
Karolina Stefanowicz
Sanja Ćavar Zeljković
Tine Drud Lundager Rasmussen
Zsuzsanna Bodi
  • Fonction : Auteur
Gugan Eswaran
Brecht Wybouw
Matthieu Bourdon
Cristina Urbez
Xiaonan Liu
Kari Salokas
Tiina Öhman
Tanya Waldie
  • Fonction : Auteur
Petri Törönen
  • Fonction : Auteur
Sedeer El-Showk
  • Fonction : Auteur
Martin Balcerowicz
Xiaomin Liu
Patrick Perkins
  • Fonction : Auteur
Serina Mazzoni-Putman
Julia Vainonen
Maija Sierla
Mikko Frilander
Susanne Mandrup
Karin Ljung
Alejandro Ferrando
Miguel Blazquez
Liisa Holm
  • Fonction : Auteur
Rupert Fray
Markku Varjosalo
Ottoline Leyser
Ville Paavilainen
Ari Pekka Mähönen
Anna Stepanova
Jose Alonso
Steffen Heber
Robert Malinowski
Finn Kirpekar
Alan Warren
Ykä Helariutta

Résumé

Polyamines are often associated with ribosomes and are thought to stabilize their integrity. In Arabidopsis , the polyamine thermospermine (tSpm) affects xylem cell fate. tSpm induces translation of SUPPRESSOR-OF-ACAULIS51 (SAC51) and SAC51-LIKEs (SACLs), which inhibit heterodimerization of the xylem development proteins LONESOME-HIGHWAY (LHW) and TARGET-OF-MONOPTEROS5. Here, we report a methyltransferase, OVERACHIEVER, that methylates the peptidyl transferase center of the 25 S ribosomal RNA (rRNA). Residue m 3 U2952 promotes functional tSpm binding to a specific site connecting the P-site transfer RNA (tRNA) with rRNA residues in the peptidyl transferase center. This interaction enhances the translation of SACLs but inhibits that of LHW. Our study uncovers the dependency between a conserved rRNA base methylation and a polyamine in orchestrating cell fate decisions, highlighting a role for the ribosome chemical landscape in translational regulation.

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hal-05508727 , version 1 (13-02-2026)

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Donghwi Ko, Raili Ruonala, Alexandre Faille, Eva Hellmann, Hanna Help, et al.. Recruitment of bifunctional regulator thermospermine to methylated ribosomes directs xylem fate. Science, 2026, 391 (6786), pp.694-699. ⟨10.1126/science.adx2867⟩. ⟨hal-05508727⟩
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