Communication Dans Un Congrès Année : 2025

First Report on the detection of novel staphylococcal enterotoxins during Staphylococcal food poisoning outbreak investigations using an in-house ELISA methods

Nawal Falou
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Résumé

Staphylococcal food poisoning outbreaks (SFPO) are some of the most common foodborne illnesses and they are a major public health concern worldwide. SFPO are caused by the ingestion of food contaminated with staphylococcal enterotoxins (SE) produced by some strains of staphylococcus aureus. To date, typing methods such as Whole Genome Sequencing (WGS) and Polymerase Chain Reaction (PCR) led to the detection of 34 SE encoding genes. However, only the so called five classical SE (SEA, SEB, SED, SED and SEE) can be detected during food poisoning outbreak (FPO) investigations and routine controls using commercial kits according to the official method EN ISO 19020. This is mainly due to the lack of specific antibodies for the detection of the non-classical SE. As a national and European reference laboratory for coagulase positive staphylococci, we develop and implement methods for the typing of S. aureus and the detection of SE during FPO investigations. During our investigations for SFPO, we often detect the presence of genes encoding for the newly described SE in isolated S. aureus strains [1, 2]. In the frame of our reference activities; we developed and implemented an enzyme-linked immunosorbent assay (ELISA) based methods for the detection of the novel SE: SEG, SEH, SEI, SEK and SEQ in food matrices. Our ELISA methods were developed using recombinant SE to produce and characterize monoclonal antibodies against these novel SEs. These ELISA methods were then used in real cases of FPO investigations in France and Ireland. Our results showed for the first time the detection of at least one novel SE investigated in food matrices involved in FPO. These results confirmed the direct implication the newly described SE in FPO, highlighting the necessity to expand the scope of SE detection for food safety applications. References: [1] Merda, D.; Felten, A.; Vingadassalon, N.; Denayer, S.; Titouche, Y.; Decastelli, L.; Hickey, B.; Kourtis, C.; Daskalov, H.; Mistou, M.-Y.; et al. NAuRA: Genomic Tool to Identify Staphylococcal Enterotoxins in Staphylococcus aureus Strains Responsible for FoodBorne Outbreaks. Front. Microbiol. 2020, 11, 1483. [2] Vingadassalon, N.; Merda, D.; Felten, A.; Chesnais, V.; Kourtis, C.; Van Nieuwenhuysen, T.; Nia, Y.; Hennekinne, J.-A.; Cavaiuolo,M. Epidemiology of Staphylococcus aureus Food Isolates: Comparison of Conventional Methods with Whole Genome Sequencing Typing Methods. Food Microbiol. 2025, 125, 104625.

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Dates et versions

hal-05486323 , version 1 (30-01-2026)

Identifiants

  • HAL Id : hal-05486323 , version 1

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Abdelhak Fatihi, Nina Aveilla, Dominique Marcé, Cécile Feraudet Tarisse, Marina Cavaiuolo, et al.. First Report on the detection of novel staphylococcal enterotoxins during Staphylococcal food poisoning outbreak investigations using an in-house ELISA methods. Food Micro Latino 2025, International Committee on Food Microbiology and Hygiene (ICFMH); Brazilian Association for Food Protection (BRAFP); Brazilian Society of Microbiology (SBM), Oct 2025, Aracaju (Brazil), Brazil. ⟨hal-05486323⟩
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