Article Dans Une Revue Nature Methods Année : 2020

Purification and enrichment of specific chromatin loci

Résumé

Understanding how chromatin is regulated is essential to fully grasp genome biology, and establishing the locus-specific protein composition is a major step toward this goal. Here we explain why the isolation and analysis of a specific chromatin segment are technically challenging, independently of the method. We then describe the published strategies and discuss their advantages and limitations. We conclude by discussing why significant technology developments are required to unambiguously describe the composition of small single loci.

Dates et versions

hal-05474219 , version 1 (23-01-2026)

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Mathilde Gauchier, Guido Van Mierlo, Michiel Vermeulen, Jérôme Déjardin. Purification and enrichment of specific chromatin loci. Nature Methods, 2020, 17 (4), pp.380-389. ⟨10.1038/s41592-020-0765-4⟩. ⟨hal-05474219⟩
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