New assessment of teichoic acids in Streptococcus pneumoniae
Résumé
TacL and LytR are thought to be responsible of the attachment of teichoic acids to the membrane (LTA) and peptidoglycan (WTA), respectively. We report using CEMOVIS the existence of a periplasmic space in S. pneumoniae WT, DeltatacL and DeltalytR strains. The observed differences in the appearance and thickness of the periplasmic space support the participation of TA to its maintenance. The sites of TA incorporation in the cell wall were examined by the metabolic incorporation of azido-choline and subsequent secondary fluorescent labeling that allowed imaging by super-resolved microscopy, to reveal consequences of the absence of TacL and LytR. In addition, a novel method of analysis by gel electrophoresis allowed the characterization of LTA and WTA in different strains and conditions. This new approach of TA analysis leads to the reassessment of previous findings.
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