Article Dans Une Revue Journal of Basic Microbiology Année : 2011

Development of a new molecular detection method for Taylorella equigenitalis

Akihiro Tazumi
  • Fonction : Auteur
Junichi Hirayama
  • Fonction : Auteur
Kyohei Hayashi
  • Fonction : Auteur
John Moore
  • Fonction : Auteur
B. Cherie Millar
  • Fonction : Auteur
Motoo Matsuda
  • Fonction : Auteur

Résumé

Abstract On PCR amplification of the intervening sequences (IVSs) in the central (helix 45) region within 23S rRNA gene sequences with T. equigenitalis ( n = 34), as well as T. asinigenitalis ( n = 35) and Bordetella ( n = 11) isolates by using the primer pair of f‐/r‐23STis2, approximately 0.8 kb of the amplicons were generated, sequenced and analyzed. One IVS of approximately 70 bp in length was identified in all the Taylorella organisms but not Bordetella. PCR amplification was further developed for the convenient and rapid molecular detection of T. equigenitalis organisms with the IVS in the helix 45 region within the 23S rRNA genes as target by using the primer pairs (f‐IVSde/r‐23de). Thus, these results clearly demonstrated that PCR amplification with the primer pair (f‐IVSde/r‐23de) can be reliable in order to differentiate the T. equigenitalis isolates from both the T. asinigenitalis and Bordetella organisms. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

Dates et versions

hal-05451333 , version 1 (09-01-2026)

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Citer

Akihiro Tazumi, Junichi Hirayama, Kyohei Hayashi, Sandrine Petry, John Moore, et al.. Development of a new molecular detection method for Taylorella equigenitalis. Journal of Basic Microbiology, 2011, 51 (3), pp.336-342. ⟨10.1002/jobm.201000309⟩. ⟨hal-05451333⟩

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