Article Dans Une Revue Lab on a Chip Année : 2025

In situ formation and culture of cell spheroids in a low-binding 3D-printed biochip

Résumé

Organ-on-chips and microfluidics systems offer new ways to overcome limitations from traditional in vitro models in preclinical studies. However, the lack of standardization and important non-specific binding of tested drugs to devices commonly made in polydimethylsiloxane (PDMS) still slow down their full integration into industrial research pipelines. The goal of this study is to develop a standardized 3D-printed biochip with low-binding properties using perfluoropolyether (PFPE), allowing long-time dynamic cultures of in situ formed cellular spheroids. We first documented the non-specific binding of molecules relevant for pharmaceutical companies, mechanical and surface properties of PFPE as compared with PDMS. A new microstructured biochip was then designed and 3D printed in PFPE to offer a 400 µL chamber containing 384 microwells. The 3D-printing fabrication protocol has been detailed considering key parameters such as UV exposure time or post-curing Finally, 384 HepG2/C3a spheroids were formed per chip in dynamic conditions and maintained for 11 days. The high viability, functionality and polarization of the spheroids cultured in these printed PFPE biochips showed the relevance of this new microphysiological system as alternative to PDMS devices.

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hal-05382340 , version 1 (25-11-2025)

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Alexandre Martins, Sylvie Klieber, Charlotte Le Graët, Eric Leclerc, Cécile Legallais, et al.. In situ formation and culture of cell spheroids in a low-binding 3D-printed biochip. Lab on a Chip, 2025, 25 (22), pp.5801-5818. ⟨10.1039/d5lc00503e⟩. ⟨hal-05382340⟩
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