Communication Dans Un Congrès Année : 2025

New NIR imaging agents based on the incorporation of lanthanide complexes in nanoparticles: applications for cancer cells detection

Résumé

Optical imaging in the near-infrared (NIR) range is of strong interest for biological research and medical diagnostic. In addition to deeper tissues penetration, emission in the NIR allows to improve both the detection sensitivity (absence of autofluorescence background) and the image resolution. The luminescence of lanthanide(III) cations (Ln3+) is attractive as they exhibit sharp emission bands, that are not affected by any environment changes (pH, temperature…). The key requirement to generate their signals is to sensitize them with a chromophore that absorb excitation light and transfer the resulting energy to the lanthanide(III). In this respect, metallacrowns (MCs) allows to coordinate and sensitize the lanthanide(III). Our lab has created several families of lanthanide(III)/zinc(II) and lanthanide(III)/gallium(III) MCs1,2,3,4. However, their use in biological system have been limited by their lack of solubility in water. Polystyrene nanoparticles (PS-NPs) are extensively utilized in biological and medical research due to their versatility, their biocompatibility and unique properties (monodispersity, tunable size, functionalization…). Recently, our lab has developed a methodology to encapsulate a large number of lanthanide-based complexes in functionalized PS-NPs through a rapid swelling process1, allowing us to incorporate a large number of MCs. Functionalized PS-NPs are used as a platform for vectorization with small molecules, peptides or antibodies. For this project, HER2-overexpressing breast cancer was taken as a model to develop targeted lanthanide-based NIR optical imaging agents. The first PS-NPs containing NIR-emitting MCs with specific HER2 targeting, addressed by the surface conjugation of a monoclonal antibodies, trastuzumab, were generated and characterized in vitro. These nanoparticles showed (i) the conservation of the specific binding properties to HER2 at the surface of cancer cells, (ii) unique photophysical properties from Ytterbium(III) ion and (iii) an absence of cytotoxicity. These nanoparticles show supplementary properties suggesting potential theranostic applications. These PS-NP are extremely promising as they constitute a versatile system that could be adapted to other targets, to multiplex and/or multimodal imaging modalities.

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Dates et versions

hal-05380110 , version 1 (24-11-2025)

Identifiants

  • HAL Id : hal-05380110 , version 1

Citer

Julie Bourseguin, Petra Cutuk, Léa Sévère, Svetlana V. Eliseeva, Timothée Lathion, et al.. New NIR imaging agents based on the incorporation of lanthanide complexes in nanoparticles: applications for cancer cells detection. FrenchBIC Annual Meeting, May 2025, Amboise, France. ⟨hal-05380110⟩
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