Poster De Conférence Année : 2025

High-Throughput Analysis of the Flagella FliK-Dependent Surfaceome and Secretome in Bacillus thuringiensis

Résumé

Bacillus thuringiensis is a entomopathogenic member of the Bacillus cereus group, known for its resistance to host antimicrobial peptides (AMPs) and high virulence in insect models. While flagella are traditionally associated with motility, they also contribute to virulence through secretion systems. This study identifies FliK, a protein involved in flagellar hook length control, as a critical determinant of AMP resistance and virulence, independent of motility. A ∆fliK mutant exhibited heightened AMP sensitivity and reduced pathogenicity in Drosophila, unlike other flagella-deficient strains. Using LC-MS/MS, we conducted a comparative secretome and surfaceome analysis of the wild-type WT and ∆fliK strains. The ∆fliK mutant showed reduced secretion of virulence factors and an increase in cell wall remodeling proteins, pointing to a broader role for FliK in protein regulation and cell surface composition. These findings highlight FliK as a key regulator in B. thuringiensis pathogenesis and AMP resistance, offering new targets for investigating bacterial immune evasion mechanisms.

Fichier principal
Vignette du fichier
poster_congres_Eupa_saint_malo_final.pdf (591.72 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Licence

Dates et versions

hal-05322292 , version 1 (20-10-2025)

Licence

Identifiants

  • HAL Id : hal-05322292 , version 1

Citer

Carine Mouawad, Mireille Kallassy Awad, Carine Rodrigues-Machado, Céline Henry, Vincent Sanchis-Borja, et al.. High-Throughput Analysis of the Flagella FliK-Dependent Surfaceome and Secretome in Bacillus thuringiensis. EuPA (European Proteomics Association), Jun 2025, Saint Malo (FR), France. ⟨hal-05322292⟩
412 Consultations
61 Téléchargements

Partager

  • More