Article Dans Une Revue Journal of Immunology Année : 2025

Genomic specificity of anti-mouse TCR mAbs determined by single-cell RNAseq

Résumé

T cells play a pivotal role in the immune system, relying on their somatically rearranged T cell receptor (TCR) to recognize peptide-MHC complexes. A comprehensive and extensively used set of monoclonal antibodies (mAbs) against TCR variable regions was generated in the previous century. The separate identification of mAb-specific TCR-V proteins and TRV genes has resulted in multiple nomenclatures, making their relationships unclear. To formally re-establish this link and determine patterns of reactivity within TRV subfamilies, we sorted T cells from C57BL/6 mice positive for any one of a panel of 22 anti-V mAbs and determined their TRV genes by single-cell TCRseq. RNAseq data revealed consistently higher expression of repeated elements from the ERV1-family LTR RLTR6Mm (mapping to Gm20400) in cells utilizing TRBV segments encoded within a 66 kb genomic region between TRBV23 and TRBV30. Our findings provide a comprehensive resource for anti-mouse TCR mAb specificity and insight into V-gene usage biases and T cell function.

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Dates et versions

hal-05304390 , version 1 (08-10-2025)

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Citer

Ian Magill, Val Piekarsa, Sofia Kossida, Josh Croteau, Amy Lee, et al.. Genomic specificity of anti-mouse TCR mAbs determined by single-cell RNAseq. Journal of Immunology, 2025, 214 (9), pp.2202-2210. ⟨10.1093/jimmun/vkaf094⟩. ⟨hal-05304390⟩
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