Communication Dans Un Congrès Année : 2025

Lipid/protein interplay in membrane formation and remodeling during plant autophagy

Julie Castets

Résumé

Autophagy is an intracellular degradation and recycling process which promotes plant acclimation and survival to a wide range of environmental stresses. During autophagy, the formation and maturation of specialized membrane vesicles, named autophagosomes, ensure cargo recognition, sequestration and trafficking to the lytic vacuole. Fusion of the autophagosomes with the vacuole delivers in its lumen a vesicle called autophagic body, which membrane is rapidly broken down thus releasing cargo for degradation. The whole dynamic process of autophagy thus relies on an orchestrated series of membrane remodeling events which underlying molecular mechanisms remain poorly understood. As essential components of biological membranes, lipids have the potential to functionally contribute to several steps of the autophagy pathway. To unravel the nature of lipids and pathways related to lipid dynamics during autophagy, we isolated autophagy compartments and established their molecular cartography. Our data highlighted the singular composition of autophagy structures compared to other endomembranes identifying novel potential determinants of membrane formation, architecture and remodeling during autophagy. Notably, we found a soluble lipid-modifying enzyme associating with all compartments of the autophagy pathway: phagophore, autophagosome and autophagic bodies. Upon autophagy inducing conditions, this enzyme which is mostly active at acidic pH, relocalizes from the cytosol to the vacuolar lumen using autophagy as trafficking system. Integrating biochemistry, cell biology and genetics, we unraveled this protein and its closest homolog as the very first actors involved in the vacuolar stages of autophagy in plant cells. Together, our results establish a model proposing that these proteins mediate the disruption of autophagic bodies as the same rate as their delivery, thus efficiently instructing the antepenultimate step of the autophagy pathway.

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Dates et versions

hal-05283163 , version 1 (25-09-2025)

Identifiants

  • HAL Id : hal-05283163 , version 1

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Julie Castets, Josselin Lupette, Matthieu Buridan, Inés Toboso Moreno, Clément Chambaud, et al.. Lipid/protein interplay in membrane formation and remodeling during plant autophagy. Jacques Monod Conference Molecular basis for membrane remodelling and organisation, May 2025, Roscoff, France. ⟨hal-05283163⟩
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