Article Dans Une Revue Electrochemistry Communications Année : 2025

Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensing

Résumé

The spatial arrangement of biorecognition molecules on the sensor surface plays a critical role in determining the performance of electrochemical biosensors. In this work, we report a covalent and tunable immobilization strategy using aryl diazonium chemistry to functionalize carbon electrodes with ethynyl groups protected by trimethylsilyl (TMS) or triisopropylsilyl (TIPS) moieties. After deprotection, an azide-modified aptamer (APT) specific to diclofenac (DCF) was immobilized via copper-catalyzed azide-alkyne cycloaddition (CuAAC). Although the TMS and TIPS groups differ in size by only 1.7 and Aring;, this small variation significantly influenced APT spacing and sensor performance. The TIPS-based sensor displayed a nearly fourfold increase in signal response compared to the TMS-based counterpart, achieving a limit of detection of 17.95 mu M. These results underscore the importance of nanoscale molecular design in optimizing label-free aptasensor sensitivity.

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hal-05246529 , version 1 (10-11-2025)

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Teodora Lupoi, Bogdan Feier, Florence Geneste, Cecilia Cristea, Yann R. Leroux. Surface organization of aptamers via diazonium grafting: A key parameter in label-free electrochemical sensing. Electrochemistry Communications, 2025, 178, pp.108000. ⟨10.1016/j.elecom.2025.108000⟩. ⟨hal-05246529⟩
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