Article Dans Une Revue Nature Communications Année : 2025

Single nucleosome imaging reveals principles of transient multiscale chromatin reorganization triggered by histone ADP-ribosylation at DNA lesions.

Junwoo Park
Edoardo José Longarini
Angela Taddei
Christian Hubert

Résumé

Timely access to DNA lesions is crucial for genome integrity. This process requires profound remodeling of densely packed chromatin to establish a repair-competent architecture. However, limited resolution has made it impossible to fully understand these remodeling events. Here, combining microirradiation with live-cell multiscale imaging, we report that DNA damage-induced changes in genome packing rely on the conformational behaviour of the chromatin fiber. Immediately after damage, a transient increase in nucleosome mobility switches chromatin from a densely-packed state to a looser conformation, making it accessible to repair. While histone poly-ADP-ribosylation is required to trigger this switch, mono-ADP-ribosylation is sufficient to maintain the open-chromatin state. The removal of these histone marks by the ARH3 hydrolase then leads to chromatin recondensation. Together, our multiscale study of chromatin dynamics establishes a global model: distinct waves of histone ADP-ribosylation control nucleosome mobility, triggering a transient breathing of chromatin, crucial for initiating the DNA damage response.

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DOI

Cite 10.5281/zenodo.13334706 Ouvrage Park, J. (2025). HTC (Version v1.0.8). Zenodo. https://doi.org/10.5281/ZENODO.13334706

DOI

Cite 10.6084/m9.figshare.28458161 Jeu de données GARCIA FERNANDEZ, F. N. (2025). Single-nucleosome imaging reveals principles of transient multiscale chromatin reorganization triggered by histone ADP-ribosylation at DNA lesions [Data set]. figshare. https://doi.org/10.6084/M9.FIGSHARE.28458161

Dates et versions

hal-05235690 , version 1 (02-09-2025)

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Fabiola García Fernández, Junwoo Park, Catherine Chapuis, Eva Pinto Jurado, Victor Imburchia, et al.. Single nucleosome imaging reveals principles of transient multiscale chromatin reorganization triggered by histone ADP-ribosylation at DNA lesions.. Nature Communications, 2025, 16 (1), pp.6652. ⟨10.1038/s41467-025-61834-7⟩. ⟨hal-05235690⟩
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