Article Dans Une Revue UTTAR PRADESH JOURNAL OF ZOOLOGY Année : 2025

Protective Effect of Portulaca oleracea L. in Cisplatin Induced Oxidative Stress in Chick Embryos: A Study with Reference to Teratogenic Deformities

Résumé

Cisplatin is a planar platinum complex consisting of two chloride leaving groups in the cis-position around platinum and is one of the most effective anticancer drugs administered to treat a variety of cancers, such as ovarian, testicular, bladder, head and neck, and uterine cervix carcinomas. Cisplatin is an active cytotoxic agent that has proved to be a successful antineoplastic agent in the treatment of various types of solid tumours. This study was conducted to study the effect of cisplatin on morphological and biochemical changes in amniotic fluid and to determine whether Portulaca oleracea L. extract can modulate these alterations at the embryonic level against cisplatin toxicity by using chick embryo as an animal model. All the results were expressed as mean ± standard error (SE) for five groups in each group, and the difference between groups was considered significant when the P value determined by unpaired Student’s t-test and less than 0.05 and 0.01 have been as indicated significant represented in astric mark in tables and figures. The morphological changes, including change in volume of amniotic fluid, body weight and alterations of amniotic fluid (AF) biochemical parameters, were studied after 24 hours of incubation by comparing cisplatin (100 µg) treated groups with their respective controls. It showed a significant dose versus time dependent reduction in volume of amniotic fluid as well as body weight of chick embryos. A dose-related increase in embryo lethality, and gross morphological deformities were observed. There was a significant change in the biochemical levels of glucose, protein, urea, creatinine, uric acid, alkaline phosphatase, alanine and aspartate transaminases with cisplatin treatment. Portulaca oleracea L. extract is also reported as an excellent source of the antioxidant vitamins such as a -tocopherol, ascorbic acid and β-carotene, as well as glutathione. It reveals to test this extract as an embryo protective agent against cisplatin-induced toxicity. In our findings, the main reason for growth retardation may be due to altered properties of the yolk sac, or extra-embryonic vascular network may reduce nutrient transfer to the embryo. Thus, the changes in the gross morphological deformities and biochemical parameters in AF with cisplatin treatment were ameliorated by pretreatment of Portulaca oleracea L. extract (1mg and 3mg) by acting as a potent protective agent at the embryonic level.

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Dates et versions

hal-05195512 , version 1 (01-08-2025)

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  • HAL Id : hal-05195512 , version 1

Citer

Sudhakar D, R Krishna Kishore. Protective Effect of Portulaca oleracea L. in Cisplatin Induced Oxidative Stress in Chick Embryos: A Study with Reference to Teratogenic Deformities. UTTAR PRADESH JOURNAL OF ZOOLOGY, 2025, 46 (14), pp.274-281. ⟨hal-05195512⟩
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