Communication Dans Un Congrès Année : 2009

Comparative analysis of IGL1 and MPS in machine perfusion, a Waters RM3 study

Résumé

Purpose: To face the growing demand for organ replacement, transplantation centers use a growing number of extended criteria grafts. These grafts are particularly sensitive to ischemia reperfusion injury (IRI). Deceased after cardiac arrest donors (DCD) grafts represent an untapped source for organs, thus it becomes critical to determine the optimal preservation strategy for these graft.
Methods: We used a Large White pig model of DCD autotransplantation to test the performance of kidney machine perfusion with two different preservation solutions: MPS, of largely extracellular composition, and IGL-1, which include intracellular components as well as a low concentration of polyethylene glycol. Kidneys undergo 60 min of warm ischemia before being stored 24h at 4°C either in cold storage (CS) or machine perfusion (MP) in a Waters RM3 machine.
Results: After 3 month, we determined that MPS MP organs, as well as IGL-1 grafts (CS or MP alike) displayed lower serum creatinine, although without reaching significance. Evaluation of proteinuria revealed that MP with MPS ameliorated graft function, an effect reproduced with IGL-1, regardless of MP or CS.
Quantitative PCR determined that MP of the graft using MPS ameliorated graft inflammation (Complement C3 and IL-17) as well as oxidative stress (NADPH oxidase element p47Phox). IGL-1 used in CS was able to reproduce that effect regarding both p47Phox and IL-17 expression. Finally, use of IGL-1 in the RM3 machine showed the most positive gene expression, with downregulation of inflammation markers TNFα, C3 and IL-17, oxidative stress marker p47Phox, and endothelial activation marker Thrombospondin.
Conclusion: We determined that machine perfusion using RM3 allowed for better chronic graft outcome on the functional and gene expression levels. Moreover, use of IGL-1 preservation solution is more efficient than MPS in maintaining graft integrity and guaranteeing long-term function.

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Dates et versions

hal-05099413 , version 1 (05-06-2025)

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  • HAL Id : hal-05099413 , version 1

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Ricardo Codas, Raphaël Thuillier, Thierry Hauet, Lionel Badet. Comparative analysis of IGL1 and MPS in machine perfusion, a Waters RM3 study. 14th ESOT Congress, The European Society Of Transplantation Congress (ESOT), Aug 2009, Paris, France. ⟨hal-05099413⟩
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