Article Dans Une Revue Plant Cell Reports Année : 2025

Designer circRNAGFP reduces GFP-abundance in Arabidopsis protoplasts in a sequence-specific manner, independent of RNAi pathways

Résumé

Circular RNAs (circRNAs) are single-stranded RNA molecules characterized by their covalently closed structure and are emerging as key regulators of cellular processes in mammals, including gene expression, protein function and immune responses. Recent evidence suggests that circRNAs also play significant roles in plants, influencing development, nutrition, biotic stress resistance, and abiotic stress tolerance. However, the potential of circRNAs to modulate target protein abundance in plants remains largely unexplored. In this study, we investigated the potential of designer circRNAs to modulate target protein abundance in plants using Arabidopsis protoplasts as a model system. We show that PEG-mediated transfection with a 50-nt circRNA GFP containing a 30-nt GFP-antisense sequence results in a dose-and sequence-dependent reduction of GFP reporter target protein abundance. Notably, a single-stranded open isoform of circRNA GFP had little effect on protein abundance, indicating the importance of the closed circular structure. Additionally, circRNA GFP also reduced GFP abundance in Arabidopsis mutants defective in RNA interference (RNAi), suggesting that circRNA activity is independent of the RNAi pathway. We also show that circRNA, unlike dsRNA, does not induce pattern-triggered immunity (PTI) in plants. Findings of this proof-of-principle study together are crucial first steps in understanding the potential of circRNAs as versatile tools for modulating gene expression and offer exciting prospects for their application in agronomy, particularly for enhancing crop traits through metabolic pathway manipulation.

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hal-05094984 , version 1 (03-06-2025)

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M Hossain, C C Pfafenrot, S S Nasfi, A Sede, J Imani, et al.. Designer circRNAGFP reduces GFP-abundance in Arabidopsis protoplasts in a sequence-specific manner, independent of RNAi pathways. Plant Cell Reports, 2025, 44, pp.128. ⟨10.1007/s00299-025-03512-y⟩. ⟨hal-05094984⟩
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