Gene re-annotations using ONT long read RNASeq data v1
Résumé
This protocol offers detailed, step-by-step instructions for anyone looking to reannotate their genes using long reads from cDNA or direct RNA RNA-Seq datasets (ONT data). The goal is not to create the perfect annotation for your organisms, but to leverage your long reads to refine gene and transcript boundaries, thereby enabling more accurate counts in single-cell or RNA-Seq experiments on non-model organisms.
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