Pré-Publication, Document De Travail Année : 2024

Dynamic change of calcium-rich compartments during coccolithophore biomineralization

Résumé

Abstract Coccolithophores are abundant marine phytoplankton that produce biomineralized calcite scales, called coccoliths, which sequester substantial amounts of carbon and play a significant role in biogeochemical cycles. However, mechanisms underlying the storage and transport of ions essential for calcification remain unresolved. We used ptychographic X-ray computed tomography under cryogenic conditions to visualize intracellular calcium-rich structures involved in the storage of calcium ions in the coccolithophore species Chrysotila carterae . During calcification, we observed a range of structures, from small electron-dense bodies within larger compartments, to denser and distributed globular compartments, before returning to small bodies once scale formation is complete. Nanobeam-scanning X-ray fluorescence measurements further revealed these electron-dense bodies are rich in P and Ca (molar ratio of ∼4:1). We infer from the dynamic nature of structures that these bodies are part of required cellular calcium ion transport pathways, a fundamental process critical for understanding the response of coccolithophores to climate change.

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hal-05051953 , version 1 (30-04-2025)

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Alexander Triccas, Daniel M. Chevrier, Mariana Verezhak, Johannes Ihli, Manuel Guizar-Sicairos, et al.. Dynamic change of calcium-rich compartments during coccolithophore biomineralization. 2025. ⟨hal-05051953⟩
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