Article Dans Une Revue International Journal of Pathogen Research Année : 2025

Bacteria Load and Antimicrobial Resistance Profiling of Bacteria Isolated from African Salad Consumed in Port Harcourt, Nigeria

Résumé

Aim: This study aims to evaluate the prevalence of antimicrobial-resistant bacteria and their resistant genes in African salad commonly consumed in West Africa. Background: The rising prevalence of foodborne infections poses a serious challenge due to the development of antimicrobial-resistant strains. Methods: The study used a cross-sectional approach to sample the food from different vendors across the two major local government areas (LGAs) in the Port Harcourt Metropolis: Obio/Akpor and Port Harcourt City. Conventional and molecular techniques were used to isolate pathogens and detect the resistance genes. Results: The findings from this study showed the most prevalent bacteria as Proteus species (30%) with Peter Odili Road (98.6±62.5 colony forming unit (CFU)/ml) as the location with the highest bacterial load while Borokiri (36.9±34.0 CFU/ml), had the lowest level of bacterial load. The microbial load based on the local government area showed that Obio/Akpor (65.6±51.7 CFU/ml) has a higher microbial load than Port Harcourt City (63.1±47.7 CFU/ml). The bacteria isolated showed the highest multiple antimicrobial resistance (MAR) indices in Woji (0.65±0.20) and the lowest indices in Ikoku (0.39±0.17). The average MAR indices of bacteria in Obio/Akpor (0.56±0.16) were marginally higher than those in Port Harcourt City Local Government (0.54±0.19). Proteus species and Providentia huaxiensis had the highest level of MAR index of 0.80. In considering the prevalence of beta-lactamase genes that were detected, there was a significant burden (p=0.0002) of the blaTEM gene observed in Obio/Akpor LGA 7 (63.4%) compared to the PHC LGA 4 (36.6%). Conclusion: Overall, antibiotic-resistant bacteria in Abacha tend to be present in remote towns with high human activities.

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Dates et versions

hal-05051166 , version 1 (29-04-2025)

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Nimi Lawson Briggs, C. Mbata, Tombari Pius Monsi, Vivian Nkemkanma Agi. Bacteria Load and Antimicrobial Resistance Profiling of Bacteria Isolated from African Salad Consumed in Port Harcourt, Nigeria. International Journal of Pathogen Research, 2025, 14 (3), pp.12-27. ⟨10.9734/ijpr/2025/v14i3359⟩. ⟨hal-05051166⟩
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