Article Dans Une Revue Science Année : 2025

In-cell architecture of the mitochondrial respiratory chain

Résumé

Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, working in concert to transfer electrons and pump protons. The precise organization of these complexes in native cells is debated. We used in situ cryo–electron tomography to visualize the native structures and organization of several major mitochondrial complexes in Chlamydomonas reinhardtii cells. ATP synthases and respiratory complexes segregate into curved and flat crista membrane domains, respectively. Respiratory complexes I, III, and IV assemble into a respirasome supercomplex, from which we determined a native 5-angstrom (Å) resolution structure showing binding of electron carrier cytochrome c . Combined with single-particle cryo–electron microscopy at 2.4-Å resolution, we model how the respiratory complexes organize inside native mitochondria.

Fichier non déposé

Dates et versions

hal-05045633 , version 1 (24-04-2025)

Identifiants

Citer

Florent Waltz, Ricardo Righetto, Lorenz Lamm, Thalia Salinas-Giegé, Ron Kelley, et al.. In-cell architecture of the mitochondrial respiratory chain. Science, 2025, 387 (6740), pp.1296-1301. ⟨10.1126/science.ads8738⟩. ⟨hal-05045633⟩
30 Consultations
0 Téléchargements

Altmetric

Partager

  • More