Poster De Conférence Année : 2025

Proteomic analyses reveal new autophagy partner involved in lipid transfer

Résumé

In plant leaves, dark stress is known to induce carbon starvation, which in turn triggers autophagy. In leaves, autophagy is used for nutrient recycling and supports energy metabolism through amino acid and lipid catabolism. The cargoes of plant autophagy are not well understood, and several research groups are investigating protein turnover in autophagy mutants to identify specific autophagy cargoes. It is known that autophagy cargoes are diverse; they may consist of oxidized proteins, aggregates, organelle portions, and components of the autophagy machinery. It has been shown in Arabidopsis thaliana that ATG8, ATG13, and ATG1 are degraded by autophagy. In our laboratory, we used LC-MS shotgun proteomics to investigate protein accumulation in the leaves of A. thaliana autophagy mutants in response to dark stress, aiming to identify potential cargoes or protein partners of the autophagy machinery. From the list of proteins that over-accumulated in autophagy mutants, we identified a candidate potentially involved in the transfer of lipids at membrane contact sites. Our Y2H and BiFC assays demonstrated that this protein interacts with ATG8. We then constructed mutants and overexpressors to investigate their phenotypes under varying nitrogen supplies, using proteomic, metabolomic, and developmental markers. Interestingly, we found that the mutants of our candidate phenocopy the autophagy mutant at different scales. Our results suggest that the protein studied is involved in the autophagy machinery and possibly in autophagosome biogenesis. Further in situ investigations and functional analyses will be performed to investigate in deep this assumption.

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Dates et versions

hal-05033200 , version 1 (14-04-2025)

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  • HAL Id : hal-05033200 , version 1

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Céline Masclaux‐daubresse, Anne Marmagne, Louis Lambret. Proteomic analyses reveal new autophagy partner involved in lipid transfer. 12th Scientific Days on Autophagy, Mar 2025, Nice, France. ⟨hal-05033200⟩
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