Article Dans Une Revue Transfusion Année : 2020

Metabolic rejuvenation upgrades circulatory functions of red blood cells stored in blood bank conditions Running head: Rejuvenation restores circulatory functions of stored RBC

Mickaël Marin
Camille Roussel
Michael Dussiot
Papa Ndour
Olivier Hermine
Yves Colin
Alan Gray
Matt Landrigan
  • Fonction : Auteur
Caroline Le van Kim
Pierre Buffet

Résumé

BACKGROUND

Red blood cells (RBC) change upon hypothermic conservation, and storage for 6 weeks is associated with the short-term clearance of 15-20% of transfused RBC. Metabolic rejuvenation applied to RBC before transfusion replenishes energetic sources and reverses most storage-related alterations, but how it impacts RBC circulatory functions has not been fully elucidated.

STUDY DESIGN AND METHODS

Six red cell concentrates (RCC) stored under blood bank conditions were analyzed weekly for six weeks, and rejuvenated on day 42 with an adenine-inosine-rich solution.

Impact of storage and rejuvenation on ATP levels, morphology, accumulation of storageinduced micro-erythrocytes (SME), elongation under an osmotic gradient (by LORRCA), hemolysis and phosphatidylserine exposure (PS) was evaluated. The impact of rejuvenation on filterability and adhesive properties of stored RBC was also assessed.

RESULTS
Rejuvenation of RCC restored intracellular ATP to almost normal levels and decreased

the PS exposure from 2.78% to 0.41%. Upon rejuvenation, the proportion of SME dropped from 28.2% to 9.5%, while the proportion of normal-shaped RBC (discocytes and echinocytes I) increased from 47.7% to 67.1%. In LORCCA experiments, rejuvenation did not modify the capacity of RBC to elongate and induced a reduction in cell volume. In functional tests, rejuvenation increased RBC filterability in a biomimetic splenic filter (+16%) and prevented their adhesion to endothelial cells (-87%).

CONCLUSION

Rejuvenation reduces the proportion of morphologically-altered and adhesive RBC that accumulate during storage. Along with the improvement in their filterability, these data show that rejuvenation improves RBC properties related to their capacity to persist in circulation after transfusion.

Fichier principal
Vignette du fichier
Marin et al, Transfusion, 2021_author copy.pdf (416.77 Ko) Télécharger le fichier

Dates et versions

hal-05031490 , version 1 (11-04-2025)

Licence

Identifiants

Citer

Mickaël Marin, Camille Roussel, Michael Dussiot, Papa Ndour, Olivier Hermine, et al.. Metabolic rejuvenation upgrades circulatory functions of red blood cells stored in blood bank conditions Running head: Rejuvenation restores circulatory functions of stored RBC. Transfusion, 2020, 61 (3), pp.903-918. ⟨10.1111/trf.16245⟩. ⟨hal-05031490⟩
39 Consultations
69 Téléchargements

Altmetric

Partager

  • More