Article Dans Une Revue Nature Communications Année : 2022

YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitro

Mireille Thomas
  • Fonction : Auteur
Elisa Dalix
  • Fonction : Auteur
Aurélien Peyron
Killian Rodriguez

Résumé

Abstract Transcriptional cofactors YAP/TAZ have recently been found to support autophagy and inflammation, which are part of cell-autonomous immunity and are critical in antibacterial defense. Here, we studied the role of YAP against Staphylococcus aureus using CRISPR/Cas9-mutated HEK293 cells and a primary cell-based organoid model. We found that S. aureus infection increases YAP transcriptional activity, which is required to reduce intracellular S. aureus replication. A 770-gene targeted transcriptomic analysis revealed that YAP upregulates genes involved in autophagy/lysosome and inflammation pathways in both infected and uninfected conditions. The YAP-TEAD transcriptional activity promotes autophagic flux and lysosomal acidification, which are then important for defense against intracellular S. aureus . Furthermore, the staphylococcal toxin C3 exoenzyme EDIN-B was found effective in preventing YAP-mediated cell-autonomous immune response. This study provides key insights on the anti- S. aureus activity of YAP, which could be conserved for defense against other intracellular bacteria.

Dates et versions

hal-05026097 , version 1 (09-04-2025)

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Citer

Robin Caire, Estelle Audoux, Mireille Thomas, Elisa Dalix, Aurélien Peyron, et al.. YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitro. Nature Communications, 2022, 13 (1), pp.6995. ⟨10.1038/s41467-022-34432-0⟩. ⟨hal-05026097⟩
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