Article Dans Une Revue Veterinary Research Année : 2025

Dynamic interplay between RNA N6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection

Zi-Han Wang
  • Fonction : Auteur
Jing Li
  • Fonction : Auteur
Sai-Ya Ma
  • Fonction : Auteur
Meng-Xuan Liu
  • Fonction : Auteur
Yu-Fei Zhan
  • Fonction : Auteur
Feng Jin
  • Fonction : Auteur
Bing-Xin Liu
  • Fonction : Auteur
Wenjing Wang
  • Fonction : Auteur
Mei He
  • Fonction : Auteur
Yu-Chuan Yang
  • Fonction : Auteur
Yandong Tang
  • Fonction : Auteur
Peng Wang
  • Fonction : Auteur
Wuchao Zhang
  • Fonction : Auteur
Jie Tong
Connectez-vous pour contacter l'auteur

Résumé

AbstractN6-methyladenosine (m6A) has attracted significant attention for its role in regulating the complex interaction between viruses and host cells. Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen affecting swine health worldwide. Here, we first identified seven m6A-enriched peaks in PRRSV genomic RNA by m6A RNA immunoprecipitation sequencing (m6A-seq). Moreover, functional analyses revealed a positive correlation between the m6A modification level and PRRSV replication. Treatment with the universal methylation inhibitor 3-deazaadenosine (3-DAA) effectively suppressed PRRSV replication in a dose-dependent manner. Furthermore, m6A-seq was also used to determine the m6A landscape of the transcriptome in PAMs infected with pandemic or highly pathogenic PRRSV strains. Among the 4677 transcripts exhibiting altered m6A modification levels, the MAPK14 gene and the p38/MAPK signalling pathway emerged as preliminary targets of m6A-mediated epigenetic regulation during PRRSV infection. These findings provide new insights into the epigenetic mechanisms underlying PRRSV infection and may facilitate the development of anti-PRRSV therapeutics.

Fichier principal
Vignette du fichier
13567_2025_Article_1495.pdf (3.23 Mo) Télécharger le fichier
Origine Publication financée par une institution
Licence

Dates et versions

hal-05002516 , version 1 (24-03-2025)

Licence

Identifiants

Citer

Zi-Han Wang, Jing Li, Sai-Ya Ma, Meng-Xuan Liu, Yu-Fei Zhan, et al.. Dynamic interplay between RNA N6-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection. Veterinary Research, 2025, 56 (1), pp.64. ⟨10.1186/s13567-025-01495-y⟩. ⟨hal-05002516⟩

Collections

39 Consultations
62 Téléchargements

Altmetric

Partager

  • More