Article Dans Une Revue Arteriosclerosis, Thrombosis, and Vascular Biology Année : 2011

Thrombospondin-1 Is a Plasmatic Marker of Peripheral Arterial Disease That Modulates Endothelial Progenitor Cell Angiogenic Properties

David Smadja
  • Fonction : Auteur
Clément d'Audigier
  • Fonction : Auteur
Ivan Bièche
  • Fonction : Auteur
Laetitia Mauge
  • Fonction : Auteur
Juliana-Vieira Dias
  • Fonction : Auteur
Julien Labreuche
  • Fonction : Auteur
Ingrid Laurendeau
  • Fonction : Auteur
Bérengère Marsac
  • Fonction : Auteur
Blandine Dizier
  • Fonction : Auteur
Orianne Wagner-Ballon
  • Fonction : Auteur
Catherine Boisson-Vidal
Verônica Morandi
  • Fonction : Auteur
Jean-Paul Duong-Van-Huyen
  • Fonction : Auteur
Patrick Bruneval
  • Fonction : Auteur
Françoise Dignat-George
Joseph Emmerich
  • Fonction : Auteur
Pascale Gaussem
  • Fonction : Auteur

Résumé

Objective— We examined whether plasma levels of angiogenic factors are altered in plasma of patients with peripheral arterial disease (PAD) and whether these factors affect endothelial progenitor cell–induced angiogenesis. Methods and Results— Plasma was collected from 184 patients with PAD and 330 age-matched healthy controls. Vascular endothelial growth factor and placental growth factor concentrations did not differ between the groups, whereas we found a linear correlation between PAD disease and thrombospondin (TSP)-1 plasma level. TSP-1 was expressed in newly formed vessels in PAD patients having received local injections of bone marrow mononuclear cells. To analyze the functional role of TSP-1 during neoangiogenesis, we used a Matrigel-plug assay and showed that vascularization of implanted Matrigel-plugs was increased in TSP-1 −/− mice. Moreover, injections of TSP-1 in C57Bl6/J mice after hindlimb ischemia induced a significant decrease of blood flow recovery. To investigate the effects of TSP-1 on human endothelial colony-forming cell (ECFC) angiogenic potential, recombinant human TSP-1 and a small interfering RNA were used. In vitro, TSP-1 N-terminal part significantly enhanced ECFC adhesion, whereas recombinant human TSP-1 had a negative effect on ECFC angiogenic potential. This effect, mediated by CD47 binding, modulated stromal cell–derived factor 1/CXC chemokine receptor 4 pathway. Conclusion— TSP-1 is a potential biomarker of PAD and ECFC-induced angiogenesis, suggesting that TSP-1 modulation might improve local tissue ischemia in this setting. (Clinical trial registration: NCT00377897.)

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Dates et versions

hal-04937934 , version 1 (10-02-2025)

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David Smadja, Clément d'Audigier, Ivan Bièche, Solène Evrard, Laetitia Mauge, et al.. Thrombospondin-1 Is a Plasmatic Marker of Peripheral Arterial Disease That Modulates Endothelial Progenitor Cell Angiogenic Properties. Arteriosclerosis, Thrombosis, and Vascular Biology, 2011, 31 (3), pp.551-559. ⟨10.1161/ATVBAHA.110.220624⟩. ⟨hal-04937934⟩
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