Article Dans Une Revue Veterinary Research Année : 2025

IFN-β production induced by PRRSV is affected by GP3 quantity control and CLND4 interaction

Dexin Li
  • Fonction : Auteur
Xinyu Cui
  • Fonction : Auteur
Yingchao Li
  • Fonction : Auteur
Qin Zhang
  • Fonction : Auteur
Hongyan Gao
  • Fonction : Auteur
Youbo Li
  • Fonction : Auteur
Yanmeng Hou
  • Fonction : Auteur
Hongjie Yuan
  • Fonction : Auteur
Yihong Xiao
Connectez-vous pour contacter l'auteur

Résumé

AbstractPorcine reproductive and respiratory syndrome virus (PRRSV) is one of the most harmful pathogens in the swine industry. Our previous studies demonstrated that the small extracellular domain (ECL2) of CLDN4 effectively blocks PRRSV infection. In this study, we explored the in vivo administration of swine ECL2 (sECL2) and found that it blocked HP-PRRSV infection and alleviated histopathological changes in organs. Notably, sECL2 stimulated cytokine production in the lungs. We observed that CLDN4 upregulated the expression of IFN-β at low doses of GP3. While high doses of GP3 inhibited the activity of the IFN-β promotor, regardless of whether CLDN4 was present. GP3 also downregulated IFN-β by decreasing the phosphorylation of TBK1 and IRF3. These findings highlight functional differences in GP3 under quantity control, which account for the variations in IFN-β induction during the early and late stages of infection. Our results indicate that sECL2 is a promising candidate drug for developing treatments to control PRRS.

Mots clés

Fichier principal
Vignette du fichier
13567_2025_Article_1455.pdf (2.35 Mo) Télécharger le fichier
Origine Publication financée par une institution
Licence

Dates et versions

hal-04925843 , version 1 (03-02-2025)

Licence

Identifiants

Citer

Dexin Li, Xinyu Cui, Yingchao Li, Qin Zhang, Hongyan Gao, et al.. IFN-β production induced by PRRSV is affected by GP3 quantity control and CLND4 interaction. Veterinary Research, 2025, 56 (1), pp.21. ⟨10.1186/s13567-025-01455-6⟩. ⟨hal-04925843⟩

Collections

59 Consultations
126 Téléchargements

Altmetric

Partager

  • More