Article Dans Une Revue Journal of Neurochemistry Année : 2005

The membrane‐associated progesterone‐binding protein 25‐Dx is expressed in brain regions involved in water homeostasis and is up‐regulated after traumatic brain injury

Brigitte Delespierre
  • Fonction : Auteur
Monique Gouézou
  • Fonction : Auteur
Philippe Leclerc
  • Fonction : Auteur
Gavin Vinson
  • Fonction : Auteur
Michael Schumacher
  • Fonction : Auteur
Donald Stein
  • Fonction : Auteur
Rachida Guennoun

Résumé

Abstract After traumatic brain injury, progesterone has important neuroprotective effects in the nervous system. There is better functional outcome and less oedema formation in pseudopregnant rat females (high levels of endogenous progesterone) than in males. In addition to intracellular progesterone receptors, membrane binding sites of the hormone such as 25‐Dx may also be involved in neuroprotection. In the present study we investigated the distribution of the membrane‐associated progesterone‐binding protein 25‐Dx in rat brain. Immunohistochemical analysis showed that 25‐Dx is particularly abundant in the hypothalamic area, circumventricular organs, and ependymal cells of the lateral walls of the third and lateral ventricles. A strong signal was also detected in the meninges. Double immunofluorescence immunolabelling and confocal microscopy showed that 25‐Dx is co‐expressed with vasopressin in neurones of the paraventricular, supraoptic and retrochiasmatic nuclei. Levels of 25‐Dx expression were higher in pseudopregnant females than in males. After traumatic brain injury, 25‐Dx expression was up‐regulated in neurones and induced in astrocytes, which play an important role in regulating water and ion homeostasis. The expression of 25‐Dx in structures involved in CSF production (choroid plexus) and in osmoregulation (circumventricular organs, hypothalamus and meninges), and its up‐regulation after brain damage, point to a novel and potentially important role of this progesterone‐binding protein in the maintenance of water homeostasis after traumatic brain injury.

Domaines

Fichier non déposé

Dates et versions

hal-04914385 , version 1 (27-01-2025)

Identifiants

Citer

Delphine Meffre, Brigitte Delespierre, Monique Gouézou, Philippe Leclerc, Gavin Vinson, et al.. The membrane‐associated progesterone‐binding protein 25‐Dx is expressed in brain regions involved in water homeostasis and is up‐regulated after traumatic brain injury. Journal of Neurochemistry, 2005, 93 (5), pp.1314-1326. ⟨10.1111/j.1471-4159.2005.03127.x⟩. ⟨hal-04914385⟩

Collections

30 Consultations
0 Téléchargements

Altmetric

Partager

  • More